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Differentiation of Two Pathogens of Powdery Mildew Disease in Flowering Dogwood (Cornus florida) by PCR-mediated Method Based on ITS Sequences |
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| Authors: | Ainong Shi Stella K Kantartzi Margaret T Mmbaga and Pengyin Chen |
| Institution: | Authors' addresses: Institute of Genomic Diversity, Cornell University, Ithaca, NY 14853, USA;;Department of Crop, Soil, and Environmental Sciences, University of Arkansas, Fayetteville, AR 72701, USA;;Otis L. Floyd Nursery Research Center, Tennessee State University, McMinnville, TN 37110, USA (correspondence to A. Shi. E-mail:;) |
| Abstract: | Two fungi, Phyllactinia guttata and Erysiphe pulchra were identified as the pathogens of powdery mildew of flowering dogwood ( Cornus florida ). The objective of this research was to identify and distinguish the two fungi by developing species-specific primers. The internal transcribed spacer (ITS) universal primers and a series of species-specific primers designed from the ITS regions were used to evaluate and validate the two fungi causing powdery mildew in dogwood. Four primer pairs showed specificity to P. guttata and three to E. pulchra . These species-specific primer pairs can be used as molecular markers to provide diagnostic tools for detection and differentiation of the two powdery mildew pathogens in flowering dogwood. |
| Keywords: | powdery mildew disease Cornus florida Phyllactinia guttata Erysiphe pulchra internal transcribed spacer polymerase chain reaction primers |