An immuno-PF2D-MS/MS proteomic approach for bacterial antigenic characterization: to Bacillus and beyond |
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| Authors: | Ruelle Virginie Falisse-Poirrier Nandini Elmoualij Benaissa Zorzi Danièle Pierard Olivier Heinen Ernst De Pauw Edwin Zorzi Willy |
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| Institution: | Center of Research on Prion Proteins, University of Liège, B-4000 Liège, Belgium. |
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| Abstract: | We are confronted daily to unknown microorganisms that have yet to be characterized, detected, and/or analyzed. We propose, in this study, a multidimensional strategy using polyclonal antibodies, consisting of a novel proteomic tool, the ProteomeLab PF2D, coupled to immunological techniques and mass spectrometry (i-PF2D-MS/MS). To evaluate this strategy, we have applied it to Bacillus subtilis, considered here as our unknown bacterial model. |
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