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HAI-1过表达对SW620细胞体外生长和运动能力的影响
引用本文:曹江 叶景佳. HAI-1过表达对SW620细胞体外生长和运动能力的影响[J]. 分子细胞生物学报, 2005, 38(3): 183-189
作者姓名:曹江 叶景佳
作者单位:浙江大学邵逸夫临床医学研究所,浙江大学肿瘤研究所 杭州 310016,杭州 310009
基金项目:国家自然科学基金(30271450),浙江省自然科学基金(300466)
摘    要:肝细胞生长因子激活因子抑制因子1(hepatocyte growth factor activator inhibitor type 1, HAI-1)能有效抑制肝细胞生长因子激活因子(hepatocyte growth factor activator,HGFA)和丝氨酸蛋白酶Matriptase的活性,并可通过对HGFA和Matriptase活性的调控参与HGF/c-Met信号传导途径。为了解HAL-1在肿瘤细胞的生长和运动中的作用,本研究将人HAI-1基因全长cDNA克隆至pcDNA3.1( )表达载体,并转染人肠癌SW620细胞,用Western blot验证了转染细胞中HAI-1的表达情况,并分别利用生长曲线、软琼脂集落形成、穿膜运动和扩散运动测定等方法检测了HAI-1 过表达对SW620细胞生长和运动能力的影响。生长曲线和软琼脂集落形成测定都显示出HAI-1 转染细胞与对照组相比差异不十分明显。穿膜运动和扩散运动测定则均显示了HAI-1过表达对细胞运动能力有明显的抑制。因此,HAI-1的过表达虽然在体外对肿瘤细胞生长影响较小,但可以抑制肿瘤细胞的运动迁移能力。

关 键 词:HAI-1  肿瘤  生长  运动
修稿时间:2004-09-20

EFFECT OF HAI-1 OVER-EXPRESSION ON IN VITRO GROWTH AND MIGRATION/MOTILITY OF SW620 CELLS
CAO Jiang,YE Jing Jia Sir Run Run Shaw Clinical Research Institute,Zhejiang University,Hangzhou ,China Cancer Institute,Zhejiang University,Hangzhou ,China. EFFECT OF HAI-1 OVER-EXPRESSION ON IN VITRO GROWTH AND MIGRATION/MOTILITY OF SW620 CELLS[J]. Journal of Molecular Cell Biology, 2005, 38(3): 183-189
Authors:CAO Jiang  YE Jing Jia Sir Run Run Shaw Clinical Research Institute  Zhejiang University  Hangzhou   China Cancer Institute  Zhejiang University  Hangzhou   China
Affiliation:CAO Jiang,YE Jing Jia Sir Run Run Shaw Clinical Research Institute,Zhejiang University,Hangzhou 310016,China Cancer Institute,Zhejiang University,Hangzhou 310009,China
Abstract:Hepatocyte growth factor activator inhibitor type 1 (HAI-1) is an effective inhibitor for hepatocyte growth factor activator (HGFA) and serine protease Matriptase, and is involved in HGF/c-Met signaling pathway by regulating activities of HGFA and Matriptase. In an attempt to elucidate the roles HAI-1 play in tumor cell growth and migration, we subcloned full length cD- NA of human HAI-1 into mammalian expression vector pcDNA3.1 ( ), and transfected the con- struct into human colorectal cancer cell SW620. The expression of HAI-1 in transfected SW620 cells was demonstrated by Western blot. Growth curve determination, soft-agar colony formation assay, trans-membrane migration assay and wound-healing cell motility assay were used to evalu- ate the effects of over-expression of HAI-1 on cell growth and migration/motility. Both of the growth curve and soft-agar colony formation assays showed that there were no significant differ- ences of growth of HAI-1-transfected cells when compared with the control groups. Trans-mem- brane migration assay and wound-healing motility assay showed significant inhibition of cell mi- gration/motility ability by the over-expressed HAI-1. Our data suggest that over-expression of HAI-1 in SW620 cells has weak effect on cell growth in vitro, but can significantly inhibit cell migration/motility.
Keywords:HAI-1  Tumor  Growth  Migration/Motility
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