Energy enzymes from the flight muscle of the house sparrow, Passer domesticus-II. Physical and chemical properties related to purification of the glycolytic enzymes

The isoelectric points (pI0), molecular weights and ammonium sulfate precipitation ranges for most of the glycolytic enzymes from house sparrow (Passer domesticus) flight muscle were determined. The pI0 for each enzyme is as follows: HK (6.8), PGI (6.7), PFK (5.4), Ald (7.2), TPI (7.5), PGK (7.1), PGM (6.1), Enol (6.2), PyK (6.6), and LDH (8.3). The molecular weight for each enzyme is as follows: PGI (145,000), Ald (160,000), TPI (60,000), PGK (35,000), PGM (60,000), Enol (100,000), PyK (200,000), and LDH (145,000). The ammonium sulfate precipitation range for each enzyme is as follows: PGI (0-80%), PFK (40-50%), Ald (40-65%), TPI (30-90%), PGK (70-90%), PGM (30-80%), Enol (45-80%), PyK (55-85%), and LDH (40-65%).