福建主要烟区烟蚜抗药性及烟蚜体内酶的活性

本文通过测定建立了灭多威、辛硫磷、吡虫啉、氰戊菊酯、高效氯氟氰菊酯和啶虫脒6种药剂的敏感基线。测定了2009年福建南平、三明和龙岩烟区的烟蚜田间种群的抗药性水平以及烟蚜种群解毒酶与靶标酶的活性。结果显示：与2008年的监测结果相比较，灭多威对福建南平、三明和龙岩3个烟区的烟蚜种群的LC50分别增长了0．004倍、0．046倍和0．051倍；辛硫磷对福建南平、三明和龙岩3个烟区的烟蚜种群的Lc50分别降低了0．213倍、0．078倍和0．088倍；毗虫啉对福建南平、三明和龙岩3个烟区的烟蚜种群的LC50分别增长了1．186倍、1．328倍和0．56倍；氰戊菊酯对南平和龙岩烟区的烟蚜种群的Lc50分别增长了0．367倍和0．139倍，对三明烟区的烟蚜种群的Lc50降低了0．103倍；高效氯氟氰菊脂对南平和龙岩烟区的烟蚜种群的LC50分别增长了0．345倍和0．244倍，对三明烟区的烟蚜种群的Lc如降低了0．035倍；啶虫脒对福建南平、三明和龙岩3个烟区的烟蚜种群的Lc靳分别增长了O．498倍、0．724倍和0．868倍。由于3个烟区近几年大田防治烟蚜极少或没有使用灭多威、辛硫磷、氰戊菊酯和高效氯氟氰菊酯这4种杀虫剂，所以3个烟区的烟蚜种群对这4种杀虫剂的抗药性增加率降低或抗药性下降。目前除了南平烟区的烟蚜种群对啶虫脒产生了高抗（抗性倍数为6cL92倍），其余烟区还未出现高抗种群。3个烟区烟蚜种群的3种酶活性差异明显，以三明种群最高，南平种群次之，龙岩种群最低。与2007年的测定结果相比较，三明、南平和龙岩的烟蚜种群CarE比活力分别增加了2．249倍、2．335倍和3．491倍；AChE比活力分别增加了0．336倍、0．664倍和0．782倍；GSTs比活力分别增加了0．591倍、0．811倍和1．447倍。

Resistance and activities of Myzus persicae (Sulzer) to insecticides in the major tobacco-planting areas of Fujian province

The sensitive baselines of tobacco aphid Myzus pers/cae （Sulzer） to methomyl, phoxim, fenvalerate, λ-cyhalothfin, imidacloprid and acetamiprid were established in this paper. The resistant levels and the activities of detoxlilcation enzymes activity and target enzyme of the aphids were determined in Nanping, Sanming and Longyan of Fujian province in 2009. Comparing with the monitoring results in 2008, the LCso of methomyl to the aphids increased 1.004, 1.046 and 1.051 times respectively in Nanping, Sanming and Longyan of Fujlan province; the LC50 of phoxlm were 0. 787, 0. 922, 0. 912 times mailer respectively; the LC50of fenvalerate increased 2. 186, 2. 328 and 1.560 times respectively; the LCso of λ-cyhalothfin increased 1. 367 and 1.139 times in Nanping and Longyan but was 0. 897 times smaller in Sanming; the LCs0 of imidaclopfid increased 1. 345 and 1. 244 times in Nanping and Longyan but was 0. 965 times smaller in Sanming; the LCs0 of aeetamiprid increased 1. 498, 1. 724 and 1. 868 times respectively. Because methomyl, phoxim, λ-eyhalothrin and imidacloprid were not used in tobacco fields of Fujian in recent years, the resistances of aphids for these pesticides tended to weaker. There were not high-resistant populations in Fujian except the aphids of Nanping with high-resistant to acetarniprid. The activities of three enzymes showed significantly different in three main planting tobacco areas. Sanming population was highest, Nanping population was high and Longyan population was low. Comparing with the data tested in 2007, the Care activity rate of aphids increased 3. 249, 3. 335 and 4. 491 times respectively in Sanming, Nanping and Longyan; the Ache activity rate increased 1. 336, 1. 664 and 1. 782 times; the GSTs activity rate increased 1. 591, 1. 811 and 2. 447 times.