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| 维吾尔药材地锦草HPLC指纹图谱的研究 | |
| 引用本文： | 谭秀芳,李晓瑾,王强,石明辉,杨杰,曹园. 维吾尔药材地锦草HPLC指纹图谱的研究[J]. 中国野生植物资源, 2011, 30(3): 47-50,68. DOI: 10.3969/j.issn.1006-9690.2011.03.013 |
| 作者姓名： | 谭秀芳 李晓瑾 王强 石明辉 杨杰 曹园 |
| 作者单位： | 1. 新疆医科大学药学院,新疆乌鲁木齐,830054 2. 新疆中药民族药研究所,新疆乌鲁木齐,830002 3. 中国药科大学中药学院,江苏南京,210009 |
| 摘 要： | 目的:建立地锦草高效液相色谱指纹图谱。方法:Shim-pack ODS C18(150 mm×4.6 mm.i.d.,5.0μm)为色谱柱,甲醇-0.1%磷酸水系统为流动相(梯度洗脱),检测波长为272 nm,柱温为30℃。结果:测定了产于新疆的10批地锦草的指纹图谱,标定了13个共有指纹峰,方法学考察结果符合指纹图谱技术要求。经计算机模拟相似度计算软件计算,相似度大于0.9。结论:建立的HPLC指纹图谱分析方法可作为地锦草的鉴别方法之一,为地锦草的质量控制提供了依据。 |
| 关 键 词： | 地锦草 高效液相 指纹图谱 |
HPLC Fingerprint Spectrum of Uighur Herba Euphorbiae Humifusae |
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| Tan Xiufang,Li Xiaojin,Wang Qiang,Shi Minghui,Yang Jie,Cao Yuan. HPLC Fingerprint Spectrum of Uighur Herba Euphorbiae Humifusae[J]. Chinese Wild Plant Resources, 2011, 30(3): 47-50,68. DOI: 10.3969/j.issn.1006-9690.2011.03.013 | |
| Authors: | Tan Xiufang Li Xiaojin Wang Qiang Shi Minghui Yang Jie Cao Yuan |
| Affiliation: | Tan Xiufang1,Li Xiaojin2,Wang Qiang3,Shi Minghui2,Yang Jie3,Cao Yuan3(1.College of Pharmacy,Xinjiang Medical University,Urmqi 830054,China,2.Chinese Herb and Minority Medicine Institute of Xinjiang,Urmqi 830000,College of Traditional Medicine,China Pharmaceutical University,Nanjing 210009,China) |
| Abstract: | Objective：To establish the HPLC fingprint spectrum of Herba Euphorbiae Humifusae identification.Methods：The chromatographic system consisted of a Shim-pack ODS C18 column（150 mm×4.6 mm.i.d.,5.0 μm） and a mobile phase of MeOH-1%H3PO4 in condition of gradient elution.The detection wavelength was 272 nm.The column temperature was30 ℃.Results：Detected the HPLC fingprint spectrum of 10 samples herbaeuphorbiae humifusae from Xinjiang;Calibrated its 13 characteristic peaks,and methodological investigation result is in accordance with the technical requirement of fingerprint spectrum.The similarity calculation software shows that the similarity is higher than 0.9.Conclusion：The HPLC fingerprint spectrum of Herba Euphorbiae Humifusae can be used as an identification method.It may provide the basis for quality control of Herba Euphorbiae Humifusae. |
| Keywords: | Herba Euphorbiae Humifusae HPLC fingerprint spectrum |
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