栽培罗汉果遗传多样性的ISSR分析

应用ISSR分子标记对62份雌株和13份雄株栽培罗汉果样品进行了遗传多样性分析,用13条ISSR引物进行扩增,共得到88条扩增带,其中64条是多态性的。计算了样品间的相似性系数,分别对雌雄株做了主成分分析,并用UPGMA法进行聚类分析。结果表明主要的栽培品种青皮果、红毛果和爆棚籽的遗传多样性很低,它们的品种内样品间相似性系数平均值分别为0.958、0.952和0.988,但有少数形态差异较大的样品具有较大的遗传差异;而茶山果和冬瓜汉具有较高的遗传多样性,品种内样品间的相似系数平均值分别为0.879和0.829;雄株也具有较高的遗传多样性。

Genetic diversity of cultivated Luohanguo(Siraitia grosvenorii)based on ISSR marker

Luohanguo( Siraitia grosvenorii ),a liana species of Cucurbitaceae,is locally economic important cultivated plant,endemically distributing in Southern,China. The genetic diversity was analyzed using inter simple sequence repeat(ISSR)technique,that a total of 62 female from eight cultivars and 13 male individuals were collected. Employed with 13 ISSR primers,88 scorable amplified bands were detected,of which 64 were polymorphic. Principal components analysis(PCA)was conducted for females and males respectively,and dendrogram was constructed using an unweighted pair group method with arithmetical averages(UPGMA)method and Nei & Li similarity coefficient. The results demonstrated that a high degree of similarity among the major cultivars Qingpiguo,Hongmaoguo and Baopengzi,with 0.958,0.952 and 0.988 of mean similarity coefficient of intra cultivars respectively,which indicated that they were low genetic diversity. However,the cultivars Chashanguo and Dongguahan showed a relatively high genetic diversity,with mean similarity coefficient of 0.879 and 0.829. The male individuals also had a high level of genetic diversity.