Molecular characterisation of Hanseniaspora species |
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Authors: | Braulio Esteve-Zarzoso María José Peris-Torán Daniel Ramón Amparo Querol |
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Affiliation: | (1) Departamento de Biotecnología, Instituto de Agroquímica y Tecnologia de Alimentos (CSIC), P.O. Box 73, E-, 46100 Burjassot, València, Spain;(2) Colección Española de Cultivos Tipo (CECT), Universitat de València, Spain |
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Abstract: | The sequences of the internal transcribed spacers (ITS regions) and the 5.8S rRNA gene, together with the electrophoretic karyotypes of 27 strains representative of the six species belonging to the genus Hanseniaspora, were examined. From the analysis of the 5.8S rRNA gene and the ITS regions, the genus Hanseniaspora is monophyletic and can be divided into two subgroups. This subdivision was supported by electrophoretic chromosome patterns. Hanseniaspora guilliermondii, H. uvarum and H. valbyensis show 6–7 bands (8 to 9 chromosomes), while the second group comprises the species H. occidentalis, H. osmophila and H. vineae which have only 5 chromosomes. |
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Keywords: | Hanseniaspora karyotypes molecular systematics RFLP 5.8S-ITS region |
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