Overexpression of cardiac actin with baculovirus is promoter dependent |
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| Authors: | Yates Susan P Otley Marissa D Dawson John F |
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| Institution: | Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ont., Canada N1G 2W1. |
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| Abstract: | The influence of the promoter and an N-terminal hexahistidine tag on human cardiac actin (ACTC) expression and function was investigated using four baculovirus constructs. It was found that both non-tagged ACTC and hisACTC expression from the p10 promoter was higher than from the polh promoter. Characterization showed that an N-terminal hexahistidine tag has a negative effect on ACTC. Recombinant ACTC inhibits DNase-I and binds myosin S1, indicative of proper folding. Our data support the hypothesis that the actin protein down-regulates the polh promoter. |
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| Keywords: | Actin Baculovirus Polyhedrin p10 |
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