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Identification of restriction endonuclease with potential ability to cleave the HSV-2 genome: Inherent potential for biosynthetic versus live recombinant microbicides |
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| Authors: | Misaki Wayengera Wilson Byarugaba |
| Institution: | (1) Restrizymes Biotherapeutics Uganda Limited, Kampala, Uganda;(2) Restrizymes Corporation- Toronto, Canada;(3) Division of Molecular Biology, Dept of Microbiology, College of Health Sciences, Makerere University, Upper Mulago Hill Road, P O Box 7072 Kampala, Uganda;(4) Division of Human and Molecular Genetics, Dept of Pathology College of Health Sciences Makerere University, Upper Mulago Hill Road, P O Box 7072 Kampala, Uganda |
| Abstract: | BackgroundHerpes Simplex virus types 1 and 2 are enveloped viruses with a linear dsDNA genome of ~120–200 kb. Genital infection with HSV-2 has been denoted as a major risk factor for acquisition and transmission of HIV-1. Developing biomedical strategies for HSV-2 prevention is thus a central strategy in reducing global HIV-1 prevalence. This paper details the protocol for the isolation of restriction endunucleases (REases) with potent activity against the HSV-2 genome and models two biomedical interventions for preventing HSV-2. |
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