柞蚕核型多角体病毒多角体蛋白基因上游5'端核苷酸序列分析

核型多角体病毒(Nuclear Polyhedrosis Virus,简称NPV)的核多角体蛋白(Polyhedrin)基因具有一个非常强的启动子和基因调控序列。目前利用这一基因的上述序列已组建了多种表达载体,高效地表达了十几种外源基因产物,成为当前最有前途的新的表达系统。但是,在组建这一病毒载体过程中,为了使插入的外源基因靠近病毒启动子序列,各

SEQUENCING OF NONCODING REGION AT 5' END OF POLYHEDRIN GENE FROM ANTHERAEA PERNYI NUCLEAR POLYHEDROSIS VIRUS ( ApNPV )

The noncoding region at the 5' end of the polyhedrin gene o?Antheraea pernyi Nuclear Polyhedrosis Virus ( ApNPV ) was sequenced. The nucleo tide sequence was compared with the corresponding region of the polyhedrin genes of Autographa colifornica NPV(ApNPV), Bombyx mori NPV(BmN- PV)and Orgyia pseudotsugata NPV (OpNPV) . The result showed that the region -l to -54bp upstream from the start codon ATG is highly con-served,the homology between them is 72% to 85%,There is a consensus se-quence CCTATAA at -3 to -9 (from -4 to -9 for ApNPV ) upstream from ATG. The nucleotides around the ATG is variable. AcNPV has a TAATG sequence, and all the genes of the four NPVs contain adenine at-3 position. An extra sequence AGATAATTA was found in AcNPV at -78 to -86bp. Our sequence data indicate that the conserved sequence at 5' end might be impor.tant for gene expression. This Information may be required for the design and construction of baculovirus expression vecter.