Identification of a disulfide-bonded 70 Kd type X procollagen in embryonic chick sternum cartilage |
| |
| Authors: | S A Jimenez V H Rao A M Reginato R Yankowski |
| |
| Affiliation: | 1. Laboratory of Immunogenetics and Molecular Biology, Federal University of Piauí, Teresina, Brazil, Campus Ministro Petrônio Portella - SG 16. Bairro Ininga, Teresina, Piauí 64049-550, Brazil;2. Department of Biology, Federal University of Piauí, Teresina, Brazil, Campus Ministro Petrônio Portella - SG 01. Bairro Ininga, Teresina, Piauí 64049-550, Brazil;3. Department of General Practice – Nephrology, Federal University of Piauí, Teresina, Brazil, Campus Ministro Petrônio Portella. Bairro Ininga, Teresina, Piauí 64049-550, Brazil;4. Universitary Hospital (HU-UFPI/EBSERH), Federal University of Piauí, Teresina, Brazil, Campus Ministro Petrônio Portella. Bairro Ininga, Teresina, Piauí 64049-550, Brazil;5. Master''s Program in Science and Health, Federal University of Piauí, Teresina, Brazil, Centro de Ciências da Saúde, Avenida Frei Serafim n° 2280. Bairro Centro, Teresina, Piauí 64001-020, Brazil;6. Graduate Program in Biotechnology - RENORBIO, Federal University of Piauí, Teresina, Brazil, Campus Ministro Petrônio Portella. Bairro Ininga, Teresina, Piauí 64049-550, Brazil;1. Department of Neurology, Hospital Clínico, Santiago de Compostela, Spain;2. Neurogenetics Research Group, Instituto de Investigaciones Sanitarias (IDIS), Santiago de Compostela, Spain;3. Fundación Pública Galega de Medicina Xenómica, Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Spain;4. Institut de Recerca Sant Joan de Déu, CIBERER, Barcelona, Spain;5. Department of Genomics and Translational Genetics, Centro de Investigación Príncipe Felipe, Valencia, Spain;6. Unit for Genetics and Genomics of Neuromuscular and Neurodegenerative Diseases, Centro de Investigación Príncipe Felipe, Valencia, Spain;7. Instituto de Biomedicina de Valencia (CSIC), CIBERER, Valencia, Spain;8. Department of Pathology, Hospital Universitario de Vigo, Spain |
| |
| Abstract: | Chondrocytes from the presumptive calcification region of 20 day old embryonic chick sternum were found to synthesize a 70 Kd Type X procollagen precursor in addition to the previously described 59 Kd Type X collagen molecules. The 70 Kd molecules exhibited an additional cyanogen bromide peptide, contained a disulfide-bonded domain, and were converted into the 59 Kd moieties during pulse-chase experiments. The conversion of the 70 Kd to the 59 Kd Type X collagen was prevented upon microtubular transport inhibition with colchicine and resulted in tissue accumulation of the 70 Kd Type X procollagen. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
