Somatic embryogenesis and organogenesis in Encephalartos dyerianus and E. natalensis |
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Authors: | Anna K. Jäger Johannes van Staden |
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Affiliation: | (1) NU Research Unit for Plant Growth and Development, Department of Botany, University of Natal Pietermaritzburg, P/BagX01, 3201 Scottsville, South Africa |
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Abstract: | Callus cultures were initiated from zygotic embryos of Encephalartos dyerianus and E. natalensis. Callus of both species were transferred onto a modified B5 medium containing different combinations of 2,4-dichlorophenoxyacetic acid and kinetin. Somatic embryogenesis and shoot organogenesis occurred in both species. The embryos were dicotyledonary. To date none of the embryos have matured.Abbreviations ABA abscisic acid - 2,4-D 2,4-dichlorophenoxyacetic acid |
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Keywords: | cycads gymnosperm in vitro culture |
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