Involvement of cationic channels in proliferation and migration of human mesenchymal stem cells |
| |
| Authors: | Fengfei Ding Guibing Zhang Lu Liu Lizhong Jiang Rui Wang Yunjie Zheng Guoping Wang Minjie Xie Yaqi Duan |
| |
| Institution: | 1. Department of Pathology, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Hangkong Road 13, Wuhan, China;2. Department of Neurology, Xiangyang Hospital Affiliated to Hubei University of Medicine, Xiangyang, Hubei, China;3. Department of Neurology, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Hangkong Road 13, Wuhan, China;4. Department of Physiology, Tongji Medical College of Huazhong University of Science and Technology, Hangkong Road 13, Wuhan, China |
| |
| Abstract: | Human mesenchymal stem cells (HMSCs) have been applied in various clinic settings. Ion channels play an important role in cellular physiology. However, the potential role of cationic channels in regulating the proliferation and migration properties of hMSCs remains to be determined. In the present study, the functional expression of ion channels in hMSCs was investigated by patch clamp. MTT assay and BrdU stainings were used to assess the proliferation of hMSCs. hMSC migration was evaluated by Transwell migration assays. The results show that sodium-, L-type calcium, potassium currents have been identified in hMSCs. TEA (K+ channel blocker), nifedipine (Ca2+ channel blocker) can inhibit both proliferation and migration of hMSCs. The increase of extracellular Ca2+ concentration promoted both proliferation and migration of hMSCs. TTX, a Na+ channel blocker, promoted cell proliferation but inhibited cell migration. Our data suggest that cationic channels (sodium, L-type calcium, potassium channels) play important roles in regulating proliferation and migration of hMSCs. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
