| 由簇毛麦V染色体引起的小麦-簇毛麦染色体代换系、易位系中线粒体蛋白质组的变化(简报) |
| |
| 引用本文： | 李纯正,尹凤英,黄海泉,王振英,彭永康,谢超杰,刘志勇,孙其信,杨作民.由簇毛麦V染色体引起的小麦-簇毛麦染色体代换系、易位系中线粒体蛋白质组的变化(简报)[J].分子细胞生物学报,2008,41(2):150-154. |
| |
| 作者姓名： | 李纯正 尹凤英 黄海泉 王振英 彭永康 谢超杰 刘志勇 孙其信 杨作民 |
| |
| 作者单位： | [1]天津师范大学细胞遗传与分子调控天津市重点实验室,天津300387 [2]中国农业大学农学与生物技术学院,北京100094 |
| |
| 基金项目： | 国家重大基础理论研究前期专项项目 |
| |
| 摘 要： | 线粒体是需氧生物中的一种半自主性细胞器.在能量代谢中,它起了一个关键的作用,柠檬酸循环、电子传递和氧化磷酸化过程均在线粒体内完成.线粒体具有高度的生物化学和遗传上的独立性.含有DNA和核糖核蛋白体.负责合成生物体内2%-5%的蛋白质1].线粒体DNA具有自身复制能力,控制着众多的遗传性状.在植物中广泛存在的细胞质雄性不育则被认为是由线粒体基因组控制的性状2].
|
| 关 键 词： | .簇毛麦 染色体代换系 易位系 线粒体蛋白质组 小麦白粉病 双向电泳 簇毛麦 染色体 小麦 代换系 易位 线粒体蛋白质 变化 简报 PROTEOME MITOCHONDRIAL CHROMOSOME EFFECT TRANSLOCATION LINE 线粒体基因组 细胞质雄性不育 存在 植物 遗传性状 控制 |
| 修稿时间： | 2007年11月2日 |
THE EFFECT OF HA YNALDIA VILLOSA V CHROMOSOME ON THE MITOCHONDRIAL PROTEOME OF WHEAT- H. VILLOSA CHROMOSOME SUBSTITUTION LINE AND TRANSLOCATION IINE |
| |
| LI Chun Zheng,HUANG Hai Quan,YIN Feng Ying,WANG Zhen Ying,PENG Yong Kang,XIE Chao Jie,LIU Zhi Yong,SUN Qi Xin,YANG Zuo Min.THE EFFECT OF HA YNALDIA VILLOSA V CHROMOSOME ON THE MITOCHONDRIAL PROTEOME OF WHEAT- H. VILLOSA CHROMOSOME SUBSTITUTION LINE AND TRANSLOCATION IINE[J].Journal of Molecular Cell Biology,2008,41(2):150-154. |
| |
| Authors: | LI Chun Zheng HUANG Hai Quan YIN Feng Ying WANG Zhen Ying PENG Yong Kang XIE Chao Jie LIU Zhi Yong SUN Qi Xin YANG Zuo Min |
| |
| Institution: | Tianjin Key Lab of Cytogenetical and Molecular Regulation, Tianjin Normal University, Tianjin 300387. |
| |
| Abstract: | Wheat-Haynaldia villosa chromosome substitution line (6A/6V) and translocation lines (6DL/6VS, 6AL/6VS) were obtained through hybridization of H. villosa with powdery mildew susceptible cultivated wheat. Substitution line and translocation lines contain V chromosome or the chromosome short arm (VS) of H. villosa. They are resistant to powdery mildew. In this study, mitochondrial proteome changes were analyzed by using substitution line (6A/6V), translocation line (6DL/6VS) as experimental materials in order to studying the effects of V chromosome on the mitochondrial proteome and related to powdery mildew resistance. The results indicated that 16 new mitochondrial protein spots (spot1, 22kDa/PI8.5; spot2, 31 kDa/PI 7.5; spot3, 28 kDa/PI 7.0; spot4, 31 kDa/PI 6.5; spot5, 40 kDa/PI 7.5; spot6, 40 kDa/PI 7.4; spot7, 80 kDa/PI 8.4; spot8, 50 kDa/PI 7.5; spot9, 60 kDa/PI 7.3; spot10, 65 kDa/PI 6.6; spot11, 65 kDa/PI 6.6; spot12, 73 kDa/PI 7.5; spot13, 73 kDa/PI 7.7; spot14, 46 kDa/PI 7.4; spot15, 46 kDa/PI 7.3; spot16, 38 kDa/PI 6.3) were produced and 7 mitochondrial protein spots (spot1, 40 kDa/PI 7.5; spot2, 43 kDa/PI 7.6; spot3, 48 kDa/PI 7.5; spot4, 42 kDa/PI 8.0; spot5, 43 kDa/PI 7.5; spot6, 32 kDa/PI 4.8; spot7, 40 kDa/PI 5.5) were absent in substitution line, 7 new mitochondrial protein spots (spotl, 43 kDa/PI 6.3; spot2, 60 kDa/PI 6.5; spot3, 60 kDa/PI 6.4; spot4, 65 kDa/PI 7.5; spot5, 55 kDa/PI 8.2; spot6, 31 kDa/PI 8.0; spot7, 43 kDa/PI 8.0) were produced and 6 mitochondrial protein spots (spot1', 66 kDa/PI 8.3; spot2', 58 kDa/PI 8.5; spot3', 36 kDa/PI 7.0; spot4', 48 kDa/PI 7.7; spot5', 48 kDa/PI 6.8; spot6', 43 kDa/PI 6.2) were absent in translocation line. These experimental results suggest that V chromosome or VS of H. villosa can obviously lead mitochondrial proteome changed. These changes may be associated with resistant to powdery mildew of substitution line and translocation line. |
| |
| Keywords: | |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
