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Diagnosis of foot-and mouth disease by real time reverse transcription polymerase chain reaction under field conditions in Brazil
Authors:Tatiane A Paixão  Alcina V Carvalho Neta  Naimes O Paiva  Jorge R Reis  Meirivan S Barbosa  Claudia V Serra  René R Silva  Tammy R Beckham  Barbara M Martin  Neville P Clarke  LGarry Adams  Renato L Santos
Affiliation:1. Departamento de Clínica e Cirurgia Veterinária, Escola de Veterinária da Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
2. Laboratório Nacional Agropecuário – Pará, Belém, PA, Brazil
3. Laboratório Nacional Agropecuário – Minas Gerais, Pedro Leopoldo, MG, Brazil
4. Texas Veterinary Medical Diagnostic Laboratory, College Station, TX, USA
5. National Veterinary Services Laboratories, U.S. Department of Agriculture, Animal Plant Health Inspection Service, Center for Veterinary Biologics, Ames, IA, USA
6. National Center of Excellence for Foreign Animal & Zoonotic Diesease Defense, College Station, TX, USA
7. Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, TX, USA
Abstract:

Background  

Indication-based data on the use of antimicrobials in animals were collected using a prospective cross-sectional survey, similarly as for surveys carried out in human medicine, but adapting the questionnaire to include veterinary-specific issues. The participating veterinarians were randomly selected from a sample population of practising veterinarians. The sampling was stratified to take into account the proportions of different types of veterinary practice in the country. All patients consulting the veterinary practice during a 1-week period were included in the study and veterinarians returned a completed questionnaire for each patient receiving antimicrobial treatment. As cattle received most of the treatments, results from the survey are given using cattle as an example species.
Keywords:
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