QuantPrime – a flexible tool for reliable high-throughput primer design for quantitative PCR |
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| Authors: | Samuel Arvidsson Miroslaw Kwasniewski Diego Mauricio Riaño-Pachón Bernd Mueller-Roeber |
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| Institution: | 1.Max-Planck Institute of Molecular Plant Physiology,Potsdam-Golm,Germany;2.Institute of Biochemistry and Biology,University of Potsdam,Potsdam-Golm,Germany;3.Department of Genetics,University of Silesia,Katowice,Poland |
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| Abstract: | Background Medium- to large-scale expression profiling using quantitative polymerase chain reaction (qPCR) assays are becoming increasingly
important in genomics research. A major bottleneck in experiment preparation is the design of specific primer pairs, where
researchers have to make several informed choices, often outside their area of expertise. Using currently available primer
design tools, several interactive decisions have to be made, resulting in lengthy design processes with varying qualities
of the assays. |
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