发展中的酵母杂交体系

酵母杂交体系包括双杂交、反向双杂交和三杂交等体系。双杂交作为一种新兴的体内研究蛋白质之间相互作用的方法，已经得到了广泛的应用。而反向双杂交和三杂交系统是在双杂交基础上发展起来的两种新技术。反向双杂交除了筛选突变株，以获取蛋白质结合的信息外，还能发现可导致已知蛋白质间特异相互作用发生解离的肽类或其他小分子物质，进一步分析蛋白质间作用位点、调控。三杂交系统则在蛋白质与小分子配基之间以及多种蛋白质之间相

Developing yeast hybrid system

Yeast hybrid systems include two-hybrid, reverse two-hybrid and three-hybrid system.Two-hybrid system is a powerful technique for detecting in vivo protein-protein interaction andbecoming wildly used in the function study of protein. Based on the two-hybrid system, other twomethods, named reverse two-hybrid and three-hybrid system, have been developed. Reverse two-hybrid system can be used to efficiently analyze mutations in interaction partners as well as for theidentification of additional trans-acting factors that dissociate macromolecular interactions. Andthe yeast three-hybrid system has a great value in discovering small ligand-receptor and multipleproteins interaction.