| 人精母细胞重组频率和年龄相关性的分析 |
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| 作者姓名： | Duan T Yang QL Wang L Shi QH Yu DX |
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| 作者单位： | 1. 安徽医科大学第一附属医院泌尿外科,合肥,230022
2. 中国科学技术大学生命科学学院分子细胞遗传学实验室,合肥微尺度物质科学国家实验室,合肥230027 |
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| 基金项目： | 国家重点基础研究发展规划(973计划)项目,中国科学院知识创新工程重要方向性项目,国家重大科学研究计划项目 |
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| 摘 要： | 减数分裂遗传重组对同源染色体的正确分离和单倍体的正确形成起至关重要的作用,但人们对人精母细胞减数分裂遗传重组机制了解的还很少。通过免疫荧光染色技术标记减数分裂I联会复合体上的MLH1(DNA错配修复蛋白)位点可以检测人精母细胞的重组。文章对10例可育男性进行分析,发现每个细胞中重组位点数平均为49.4士4.4,范围为33~63,具有显著的个体差异,只有0.4%(1/220)的常染色体SC上缺少MLH1位点。进一步通过Spearman相关性分析,分析了年龄因素与个体间重组位点差异的相关性,结果提示年龄因素对常染色体及性染色体的重组均无影响。
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| 关 键 词： | 减数分裂 联会复合体 遗传重组 精母细胞 年龄 |
Correlation analysis between meiotic recombination frequencies and age in human spermatocyte |
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| Duan T,Yang QL,Wang L,Shi QH,Yu DX.Correlation analysis between meiotic recombination frequencies and age in human spermatocyte[J].Hereditas,2011,33(7):725-730. |
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| Authors: | Duan Tao Yang Qing-Ling Wang Liu Shi Qing-Hua Yu De-Xin |
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| Institution: | DUAN Tao1,YANG Qing-Ling2,WANG Liu2,SHI Qing-Hua2,YU De-Xin1 1.Department of Urology,First Affiliated Hospital,Anhui Medical University,Hefei 230022,China,2.Hefei National Laboratory for Physical Sciences at Microscale,School of Life Science,University of Science Technology of China,Hefei 230027 |
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| Abstract: | Faithful meiotic recombination is essential for the segregation of homologous chromosomes and the formation of normal haploid gametes.Little is known about the mechanism of meiotic recombination in human germ cells.MLH1(a DNA mismatch repair protein) foci on synaptonemal complexes(SCs) at prophase I of meiosis can be used to examine re-combination frequency.In 10 fertile men,the mean number of MLH1 foci per cell in all donors was 49.4 with a range from 33 to 63.There was significant variation in the recombi... |
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| Keywords: | meiosis synaptonemal complex genetic recombination spermatocyte age |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ |
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