| 鸡L-FABP基因全长cDNA克隆表达及与杂种鸡脂肪沉积的关系 |
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| 引用本文： | Yu Y,Wang D,Sun DX,Xu GY,Li JY,Zhang Y. 鸡L-FABP基因全长cDNA克隆表达及与杂种鸡脂肪沉积的关系[J]. 遗传, 2011, 33(7): 763-767. DOI: 10.3724/SP.J.1005.2011.00763 |
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| 作者姓名： | Yu Y Wang D Sun DX Xu GY Li JY Zhang Y |
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| 作者单位： | 1. 中国农业大学动物科技学院,北京,100193
2. 中国农业大学动物科技学院,北京100193;中国农业科学院北京畜牧兽医研究所,北京100193 |
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| 基金项目： | 国家重点基础研究发展规划项目,转基因重大专项,教育部基本科研业务项目 |
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| 摘 要： | 肝脏脂肪酸结合蛋白(L-FABP)与脂肪运输及沉积关系密切。文章以8周龄丝羽乌鸡(CC)、农大褐蛋鸡(DD)及其正反杂交组合鸡(CD和DC)为试验材料,利用mRNA差异显示技术,在肝脏组织中获得一条阳性差异表达片段。通过片段回收、测序及序列比对发现,该差异表达片段为鸡L-FABP基因的全长cDNA编码序列(NCBI登录号:AY321365)。Northern杂交和半定量RT-PCR结果显示,该基因在正反杂交组合CD及DC的肝脏组织中表达量均明显高于亲本CC和DD,与杂种鸡的高腹脂及较大肌间脂宽表型趋势相同。鉴于L-FABP基因的高表达可能导致杂种鸡的脂肪沉积高于亲本,有必要针对鸡L-FABP基因进行深入的功能研究。
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| 关 键 词： | L-FABP 脂肪性状 丝羽乌鸡 农大褐蛋鸡 正反杂交组合 |
Whole cDNA sequence cloning and expression of chicken L-FABP gene and its relationship with lipid deposition of hybrid chickens |
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| Yu Ying,Wang Dong,Sun Dong-Xiao,Xu Gui-Yun,Li Jun-Ying,Zhang Yuan. Whole cDNA sequence cloning and expression of chicken L-FABP gene and its relationship with lipid deposition of hybrid chickens[J]. Hereditas, 2011, 33(7): 763-767. DOI: 10.3724/SP.J.1005.2011.00763 |
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| Authors: | Yu Ying Wang Dong Sun Dong-Xiao Xu Gui-Yun Li Jun-Ying Zhang Yuan |
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| Affiliation: | YU Ying1,WANG Dong1,2,SUN Dong-Xiao1,XU Gui-Yun1,LI Jun-Ying1,ZHANG Yuan1 1.College of Animal Science and Technology,China Agricultural University,Beijing 100193,China,2.Institute of Animal Sciences,Chinese Academy of Agricultural Sciences |
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| Abstract: | Liver fatty acid-binding protein(L-FABP) is closely related to intracellular transportation and deposition of lipids.A positive differential displayed fragment was found in the liver tissue among Silkie(CC),CAU-brown chicken(CD),and their reciprocal hybrids(CD and DC) at 8 weeks-old using differential display RT-PCR techniques(DDRT-PCR).Through recycling,sequencing,and alignment analysis,the fragment was identified as chicken liver fatty acid-binding pro-tein gene(L-FABP,GenBank accession number AY321365).R... |
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| Keywords: | L-FABP |
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