Pyrimidine dimer dependent cleavage of single-stranded DNA by T4 UV endonuclease |
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| Authors: | W Sauerbier |
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| Affiliation: | 1. Project Research Center for Nosocomial Infectious Diseases, Hiroshima University, Hiroshima, Japan;2. Department of Infectious Diseases, Hiroshima University Hospital, Hiroshima, Japan;3. Department of Surgery, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan;4. Department of Virology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan;5. Translational Research Center, Hiroshima University, Hiroshima, Japan;1. Center for Environmental Research and Children''s Health (CERCH), School of Public Health, University of California at Berkeley, Berkeley, CA, USA;2. Department of Laboratory Medicine, University of Milano-Bicocca, School of Medicine, Hospital of Desio, Desio-Milano, Italy;1. Department of Infectious Diseases, West German Centre of Infectious Diseases, Universitätsmedizin Essen, University Duisburg-Essen, Germany;2. Institute for Virology, University Hospital Essen, University of Duisburg-Essen, Essen, Germany;3. Hust Huazhong University of Science & Technology, Wuhan Union Hospital, Department of Infectious Diseases, Wuhan, China;4. Wuhan-Essen Joint International Laboratory of Infection and Immunity, Essen, Germany;5. Department of Molecular and Medical Virology, Faculty of Medicine, Ruhr University Bochum, Bochum, Germany |
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| Abstract: | T4 UV endonuclease cleaves double- and single-stranded DNA with equal specificity for photo-pyrimidine dimers. Thus, the enzyme can be used for mapping and quantifying pyrimidine dimers in single-stranded DNA as well as in double-stranded DNA. Mapping of pyrimidine dimers shows that rates of UV-dimerization are not only affected by 5', 3' adjacent bases, but also by position within pyrimidine tracts. Di-pyrimidines at 3' ends of tracts are more photoreactive than those at 5' ends. |
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