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Capacitance--voltage relationship in phospholipid bilayers containing gangliosides |
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| Authors: | C Usai C Marchetti F Gambale M Robello A Gorio |
| Affiliation: | 1. Istituto di Cibernetica e Biofisica, CNR, Corso Mazzini 20, 16032 Camogli, Genova, Italy;2. FIDIA Research Laboratories, Via Ponte della Fabbrica 3a, 35031 Abano Terme, Padova, Italy;3. Istituto di Scienze Fisiche, Università di Genova, viale Benedetto XV, 5, 16132 Genova, Italy |
| Abstract: | Changes in the position of the minimum of the parabolic capacitance-voltage curve allow the measurement of the amount of ganglioside present in artificial bilayers made with phosphatidylcholine-ganglioside mixtures and asymmetrically shielded with Ca2+. The screening effect of the ionic solution must be considered. With ganglioside/phospholipid molar ratios of up to 15%, all glycolipids can be found at the membrane surfaces. |
| Keywords: | Ganglioside Lipid bilayer Membrane capacitance Surface charge BLM black lipid membranes DOPC dioleoylphosphatidylcholine PS phosphatidylserine Galβ1→3GalNAcβ1→4Gal (3←2αNeuAc) β1→4Glcβ1→1Cer NeuAcα2→3Galβ1→ 3GalNAcβ1→4Gal (3←2αNeuAc)β1→4Glcβ1→ 1Cer GT NeuAcα2→3Galβ1→3GalNAcβ1→ 4Gal(3←2αNeuAc8←2αNeuAc)β1→4Glcβ1→ 1Cer (abbreviations recommended by Svennerholm [1]) |
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