Protoplast formation and cell wall regeneration inClostridium
thermocellum |
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Authors: | Kathy L Gottlund Bland S Montenecourt Jeffrey A Sands |
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Institution: | (1) Molecular Biology Program Center for Molecular Bioscience and Biotechnology, Lehigh University, 18015 Bethlehem, PA, USA |
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Abstract: | Summary A protocol was developed for the production ofClostridium
thermocellum protoplasts and the regeneration of protoplasts into vegetative cells. Protoplasts were prepared by exposure of whole cells to lysozyme (125 ug/ml for 10 min. at 45 C). Protoplasts plated on regeneration agar reverted to walled cells with a frequency of up to approximately 0.3 % of input cells. |
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