A novel, inexpensive, and sensitive method for analysis of tyrosine hydroxylase activity in tissue samples. |
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Authors: | C L Blank R Pike |
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Institution: | Department of Chemistry, University of Oklahoma, Norman, OK 73069, USA |
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Abstract: | Tyrosine hydroxylase activity in whole mouse brains was measured . The L-dihydroxyphenylaline (L-DOPA) formed by the enzyme was quantitated by liquid chromatography with electrochemical detection (LCEC). An investigation of the incubation factors (added Fe+2, DOPA decarboxylase inhibitor concentration, substrate concentration, amount of tissue, time of incubation) is reported. Under optimal conditions the activity was found to be 15.1 ± 0.6 (S.E.M.) nmol DOPA formed/hr./g. tissue. |
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