A new cuticle scale hydrolysing protease from Beauveria brongniartii |
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| Authors: | A Erlacher F Sousa M Schroeder S Jus V Kokol A Cavaco-Paulo G M Guebitz |
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| Institution: | (1) Department of Environmental Biotechnology, Graz University of Technology, Petersgasse 12, Graz, Austria;(2) Textile Department, University of Maribor, Maribor, Slovenia;(3) Department of Textile Engineering, University of Minho, Guimaraes, Portugal |
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| Abstract: | From a screening for the production of new proteases specific for cuticle scales, Beauveria brongniartii was selected producing an alkaline Ca++ dependent protease. The purified had a molecular weight of 27 kDa and a pI value of 8.0. Substrate specificities of model substrates (wool with partially removed cuticles treated with SDS) were analyzed by protein release, dissolved organic carbon (DOC) and nitrogen analysis. The C/N ratio of released material turned out to be a good parameter to determine the site of action of proteases on fibres. Compared to other enzymes, the fungal protease preferentially hydrolyzed cuticle scales and has thus a potential for anti-shrinking pre-treatment of wool fabrics. |
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| Keywords: | Wool Cuticle Protease Beauveria brongniartii |
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