Coat protein conformation in M13 filaments,I-forms and spheroids |
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Authors: | Marcia Manning Marsha Moore Linda Spremulli Jack Griffith |
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Institution: | 1. Cancer Research Center University of North Carolina Chapel Hill, North Carolina 27514 USA;2. Department of Bacteriology and Immunology University of North Carolina Chapel Hill, North Carolina 27514 USA;3. Department of Biochemistry and Nutrition University of North Carolina Chapel Hill, North Carolina 27514 USA;4. Department of Chemistry University of North Carolina Chapel Hill, North Carolina 27514 USA |
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Abstract: | Circular dichroism studies of the filamentous coliphage M13 were carried out to determine conformational changes in the major capsid protein (the B protein) that occur during contraction of the filaments to I-forms and spheroids. The α-helicity of the B protein is somewhat lower in the I-forms than in filaments and much lower in spheroids. This conformational change may explain the increased detergent and lipid solubility of both I-forms and spheroids relative to filaments. |
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