S-acylated residues of the acyl-carrier protein subunit of Klebsiella, aerogenes citrate lyase

Oxidation of the isolated deacetyl acyl-carrier protein subunit of citrate lyase from $\text{Klebsiella}\text{,}\text{aerogenes}$ with Cu²⁺-$\text{o}$-phenanthroline complex leads exclusively to intrapeptide disulfide bridge formation indicating that the cysteamine and the cysteine residues are located in close proximity. The S-acetylation of the cysteine residue in deacetyl acyl-carrier protein subunit is catalysed by a citrate lyase ligase preparation in presence of acetate and ATP. Reaction-inactivation of citrate lyase results in deacetylation of the S-acetyl cysteamine residue of the prosthetic group but not of the S-acylated cysteine residue in the acyl-carrier protein.