Identification of two cDNA clones coding for androgen-dependent polypeptides in rat ventral prostate

cDNA clones were obtained by transformation of $\text{E. coli}$ x1776 with pBR322 containing insert of ds cDNA synthesized from total rat prostate poly(A) RNA. Two prostate-specific cDNA clones were isolated by colony hybridization and identified by message selection/translation as encoding polypeptides of M_r: 13,500 and 9,300. Hybridization of poly(A) RNA from normal and castrated rat prostates to the cloned cDNAs indicated that the levels of mRNAs coding for M_r: 13,500 and 9,300 polypeptides are regulated by testosterone.