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Leumorphin is a novel endogenous opioid peptide derived from preproenkephalin B |
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| Authors: | Kazuwa Nakao Mitsuaki Suda Makoto Sakamoto Takaaki Yoshimasa Narito Morii Yoshio Ikeda Chizuko Yanaihara Noboru Yanaihara Shosaku Numa Hiroo Imura |
| Institution: | 1. 2nd Division, Department of Medicine, Kyoto University School of Medicine, Kyoto 606, Japan;2. Department of Medical Chemistry , Kyoto University School of Medicine, Kyoto 606, Japan;3. Shizuoka College of Pharmacy, Shizuoka , Japan;4. National Institute for Physiological Sciences, Okazaki , Japan |
| Abstract: | Using synthetic leumorphin, we obtained antisera for leumorphin and set up two radioimmunoassays (RIAs) with different specificities. Gel exclusion chromatography coupled with the two RIAs showed the existence of a considerable amount of leumorphin-like peptide in water extracts from porcine neurointermediate pituitaries. Reverse phase high performance liquid chromatography revealed that leumorphin-like peptide in the water extracts was indistinguishable from synthetic leumorphin. These results along with potent opioid activity of leumorphin indicate that leumorphin is a novel endogenous opioid peptide derived from preproenkephalin B. |
| Keywords: | leumorphin Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Gln-Phe-Lys-Val-Val-Thr-Arg-Ser-Gln-Glu-Asp-Pro-Asn-Ala-Tyr-Tyr-Glu-Glu-Leu-Phe-Asp-Val HPLC high performance liquid chromatography RIA radioimmunoassay EDTA Ethylenediaminetetraacetic acid LI like immunoreactivity |
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