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Exit of proteins and fragments thereof from mitochondria is accelerated by the import of cytosolic synthesized proteins
Authors:José Hernández-Yago  Erwin Knecht  Vicente Felipo  Vicente Miralles  Santiago Grisolía
Institution:Instituto de Investigaciones Citológicas de la Caja de Ahorros de Valencia Amadeo de Saboya, 4 Valencia -10- Spain
Abstract:Most mitochondrial proteins are synthesized on cytosolic ribosomes and imported into mitochondria. Incubation of 35S-methionine labeled mitochondria from rat hepatocytes with proteins synthesized in a cell-free system, using messenger RNA from rat liver, dramatically increased the release of mitochondrial proteins and fragments thereof into the medium. Since the synthesized proteins include cytosolic precursors of mitochondrial proteins, our results strongly suggest that import of proteins from the cytosol into mitochondria influences the half-life of proteins in these organelles. The use of this simple approach — i.e. combining the study of protein import and exit with mitochondria — to further clarify intracellular protein turnover and its regulation is suggested.
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