Screening cDNA expression libraries with monoclonal and polyclonal antibodies using an amplified biotin-avidin-peroxidase technique |
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| Authors: | B T French H M Maul G G Maul |
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| Institution: | 1. CVAS, Jhang Campus, University of Veterinary and Animal Sciences, Lahore, Pakistan;2. Department of Biochemistry, Government College University, Faisalabad, Pakistan;3. Institute of Chemistry, Shah Abdul Latif University, Khairpur 66020, Sindh, Pakistan;4. Department of Chemistry, University of Agriculture, Faisalabad, Pakistan;5. Department of Chemistry, The University of Lahore, Lahore, Pakistan;1. Division of Medical Toxicology, Department of Emergency Medicine, UC San Diego Health, CA;2. VA San Diego Healthcare System, San Diego, CA, United States of America;3. San Diego Division, California Poison Control System, San Diego, CA, United States of America;4. Department of Emergency Medicine, UC San Diego Health, CA, United States of America |
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| Abstract: | A chromogenic method using biotinylated secondary antibodies and peroxidase-coupled avidin for screening cDNA expression libraries is described. This method offers increased sensitivity over peroxidase-coupled secondary antibodies and rapid processing of samples, and avoids preparation and handling of radioactive materials. All materials for the chromogenic assay are available commercially and the method offers a fast and easy way to screen lambda and plasmid expression libraries with mono- and polyclonal antibodies. |
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