Molecular analysis of Ascochyta rabiei (Pass.) Labr., the pathogen of ascochyta blight in chickpea |
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| Authors: | D K Santra G Singh W J Kaiser V S Gupta P K Ranjekar F J Muehlbauer |
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| Institution: | (1) U.S. Department of Agriculture, Agricultural Research Service, 303 W Johnson Hall, Washington State University, Pullman, WA 99164-6434, USA e-mail: muehlbau@wsu.edu Tel.: +1-509-335 9521, Fax: +1-509-335 7692, US;(2) Department of Plant Breeding and Genetics, Punjab Agricultural University. Ludhiana, Punjab, India, IN;(3) Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, India, IN |
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| Abstract: | Genetic diversity in Ascochyta rabiei (Pass.) Labr., the causative agent of ascochyta blight of chickpea, was determined using 37 Indian, five American (USA),
three Syrian, and two Pakistani isolates. A total of 48 polymorphic RAPD markers were scored for each isolate and the data
used for cluster analysis. Most of the isolates clustered in the dendrogram essentially according to geographic origin. Based
on the two major clusters A and B, Indian isolates were grouped into two categories, type-A and type-B. Isolates of A. rabiei within the Punjab state were more diverse than isolates from other states in northwestern India. A DNA marker (ubc7561.6 kb), specific to Indian isolates was identified. This is the first report of a molecular diversity analysis of Indian isolates
of A. rabiei. The information may assist Indian chickpea breeders in the proper deployment of blight-resistant cultivars and in disease
management.
Received: 25 April 2000 / Accepted: 11 July 2000 |
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| Keywords: | RAPD markers Genetic diversity Phytopathogen |
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