Occupied and unoccupied type II estrogen binding sites in human breast cancer |
| |
| Authors: | M T Lopes M H Liberato A Widman M M Brentani |
| |
| Affiliation: | 1. Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil;4. AGAP, Univ. Montpellier, CIRAD, INRA, Montpellier SupAgro, Montpellier, France;5. Centro de Pesquisa Agropecuária do Trópico Semiárido, Empresa Brasileira de Pesquisa Agropecuária, Petrolina, Brazil;1. Department of Endocrinology, Nanjing Lishui District Hospital of Traditional Chinese Medicine, Nanjing, 211200, China;2. Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 210028, China;3. Laboratory of Translational Medicine, Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, 210028, China;4. Department of TCMs Pharmaceuticals, School of TCM & State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, China;1. Dipartimento di Ingegneria, Università degli Studi di Palermo, viale delle Scienze ed. 6, 90128 Palermo, Italy;2. Medtronic, via Camurana 1, 41037 Mirandola, Modena, Italy |
| |
| Abstract: | Using a saturation analysis over a wide range of [3H]estradiol at two temperatures 4 and 22 degrees C we have determined unoccupied (4 degrees C) and total (22 degrees C) type II estrogen binding site (EBS) levels in individual cytosols of 100 patients with breast cancer (50 post and 50 premenopausal). Exchange was found to be complete after 18 h at 22 degrees C and receptor degradation was negligible during this treatment. Steroid specificity and affinity determined by Scatchard and Rosenthal plot analysis were not altered at 22 degrees C. Carcinomas presented a higher total type II REBS level as compared unfilled type II binding sites or the classical ER, independently of menopausal status, phase of the menstrual cycle or positivity of ER. On the other hand, unoccupied type II EBS level was strongly correlated to the concentration of type I ER, being higher on the post-menopausal group and older patients. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
