Cloning of a DNA region of aPseudomonas plasmid that codes for detoxification of the herbicide paraquat |
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| Authors: | Dr Mohd Azib Salleh John M Pemberton |
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| Institution: | (1) Department of Genetics, National University of Malaysia, Bangi, Selangor, Malaysia;(2) Department of Microbiology, University of Queensland, Brisbane, Australia |
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| Abstract: | A newly isolatedPseudomonas plasmid coding for detoxification of the herbicide paraquat (Pqr) was characterized. AnEcoR1-generated fragment derived from the plasmid carrying the Pqr determinant was cloned intoEscherichia coli. Subsequent subclonings, followed by exonuclease III-mediated deletion analysis, localized the Pqr gene(s) to a 1.8-kb segment within a 4.2Pst1 subfragment. The cloning and apparent expression of the Pqr gene(s) inE. coli will enable its structural organization and function to be analyzed in detail. |
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