鲍曼不动杆菌整合子分型与耐药性的初步研究

目的了解临床分离的43株鲍曼不动杆菌中Ⅰ类、Ⅱ类、Ⅲ类整合子的流行病学现状及其耐药性。方法用多重PCR方法扩增30株鲍曼不动杆菌Ⅰ类、Ⅱ类、Ⅲ类整合酶基因，用K—B法检测鲍曼不动杆菌的耐药情况。结果Ⅰ类整合子检出率为79．1％（34／43），未检出Ⅱ、Ⅲ类整合子。结论Ⅰ类整合子阳性的菌株的耐药率较高，显著高于Ⅰ类整合子阴性的菌株，多重PCR是筛查革兰阴性杆菌整合子的有效方法。

Classification of integron of Acinetobacter baumanii and its resistance analysis

Objective To investigate the distribution of class 1 ,class 2 and class 3 integrons in the Acinetobacter baumanii isolated from the First Affiliated Hospital of Sun Yat-sen University and analyze the correlation between integrons and its resistance. Method Total 43 strains of Acinetobacter baumanii were coUected. Multiple-PCR method was used for screening of class 1, class 2 and class 3 integrons. K-B method was used to determine the drug sensitivity to antibiotics. Result The incidence of class 1 integron was 79.1% while class 2 and 3 integron were not detected among 43 strains of Acinetobacter baumanii. Conclusion The incidence rate of class 1 integron was quite high in Acinetobacter baumanii. The drug resistance of the positive strains of class 1 integron was significantly higher than that of negative strains.