中国淡水涡虫RAPD分析条件的优化（简报）

涡虫是动物界最早出现两侧对称、三胚层、营自由生活的扁形动物，也是由水生向陆生过渡的重要类群，因而在动物系统演化中占有重要地位。由于涡虫再生能力极强，因此成为研究细胞分化与去分化分子机理的理想材料，近百年来一直是国际动物学界热衷探索的研究领域之一。然而遗憾的是，我国动物学界从事涡虫研究者甚少，属于基础十分薄弱的

Optimization of reaction conditions for RAPD analysis of freshwater planarians in China]

Using the Genomic DNA purification kit, the total DNA of the freshwater planarian was extracted and developed one single band through 0.8% agarose gel electrophoresis with OD260/OD280 between 1.5 and 2.2, which could satisfy the requirements of RAPD and PCR on DNA. With the extracted DNA template, we tested experimental conditions that might affect RAPD results including annealing temperature, concentrations of template DNA, primer, Mg2+ and dNTPs. Through comparision we found that it was necessary and important to optimize the experimental conditions for producing stable and repeatable RAPD results. The optimized reaction conditions of RAPD for freshwater planarian in 25 microl reaction volume were as follows: 20 ng template DNA, 37 degrees C annealing temperature, 0.2 micromol/L primer, 2.0 mmol/L Mg2+ and 200 micromol/L dNTPs.