变形链球菌致龋抗原基因在乳酸乳球菌的表达

目的 克隆变形链球菌葡聚糖结合蛋白B(GbpB)功能区的基因片段,并在乳酸乳球菌中表达.方法 在实验中利用了分子克隆技术构建携带GbpB基因的重组原核表达质粒pNI1,将重组质粒转化乳酸乳球菌YF02株,筛选鉴定阳性菌落,诱导表达的GbpB蛋白用SDS-PAGE进行鉴定.结果 成功克隆了GbpB功能区的基因片段,并在乳酸乳球菌中得到其融合蛋白的表达.结论 利用分子生物学技术能够成功克隆GbpB功能区基因并获得乳酸乳球菌融合蛋白的表达,为后续研究奠定了基础.

Cloning of the functional domains of Streptococcus mutans glucan-binding protein B and it's expressiong in Lactoboccus lactis

Objective To clone the functional domains of Streptococcus mutans Glucan-Binding Protein B (GbpB) and express it's fusion protein in Lactoboccus lactis. Method First, the GbpB gene of Streptococcus mutans was cloned into the prokaryotic espressive vector pNI1. Second, the recombinant vector GbpB-pNI1 was transformated into Lactoboccus lactis strain YF02 to express GbpB protein. Then the recombinant GbpB was induced to express and was identified by SDS-PAGE.Result The functional domains of Glucan-Binding Protein B was cloned correctly and it's fusion protein was expressed in Lactoboccus lactis.Conclusion PCR(polymerase chain reaction) and construction of fusion protein technique are effective methods to get the aimed genes and it's fusion protein. This experiment has provided a base to the further research.