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Ovomucoid partitioning in aqueous two-phase systems
Authors:Fabí  ola Cristina de Oliveira, Jane S  lia dos Reis Coimbra, Luis Henrique Mendes da Silva, Edwin Elard Garcia Rojas,Maria do Carmo Hespanhol da Silva
Affiliation:aDepartment of Food Technology, Federal University of Viçosa (UFV), P. H. Rolfs Av., s/n, 36570-000 Viçosa, MG, Brazil;bDepartment of Chemistry, Federal University of Viçosa (UFV), P. H. Rolfs Av., s/n, 36570-000 Viçosa, MG, Brazil;cDepartment of Agribusiness and Administration Engineering, Federal University Fluminense (UFF), Trabalhadores Av. 420, 27225-250 Volta Redonda, RJ, Brazil
Abstract:This study evaluated the partitioning of ovomucoid from egg white, in aqueous two-phase systems (ATPS) composed of PEG 1500 and inorganic salt (lithium sulfate, sodium sulfate, magnesium sulfate, sodium carbonate or sodium citrate) at 25 °C. The results showed a great effect of the electrolyte nature on the partition coefficient. The partition coefficient value ranges from 0.02 to 6.0. The highest partition coefficients were obtained from systems composed of sodium carbonate and the lowest in systems composed of magnesium sulfate. In the system containing magnesium sulfate, a recovery percentage greater than 90% was obtained.
Keywords:Aqueous two-phase   Extraction   Bioseparation   Partitioning   Egg white   Ovomucoid
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