Molecular cloning of cDNA for rat liver catalase |
| |
Authors: | T Osumi H Ozasa S Miyazawa T Hashimoto |
| |
Affiliation: | Department of Biochemistry, Shinshu University School of Medicine, Matsumoto, Nagano 390, Japan |
| |
Abstract: | For the studies on the induction of peroxisomal enzymes by hypolipidemic agents, we have tried to isolate a cDNA clone for rat liver catalase. A recombinant clone, pMJ501, was isolated, of which cDNA insert specifically hybridized to catalase mRNA in hybridization-selected translation. On RNA blot hybridization, it hybridized to 2.4-kilobases RNA which was increased about 1.5-fold by the administration of di-(2-ethylhexyl)phthalate to the rats. The nucleotide sequence of the cDNA contains a reading frame for 109 amino acid residues which match the reported amino acid sequence of bovine liver catalase at the carboxyl end with 82% homology. It is concluded that pMJ501 contains a cDNA sequence for rat liver catalase. |
| |
Keywords: | |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|