Uptake of chromium(III) and chromium(VI) compounds in the yeast cell structure |
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Authors: | Maja Pa?: Radmila Mila?i? Kazimir Dra?ar Nata?a Pollak Peter Raspor |
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Institution: | (1) Biotechnical Faculty, Food Science and Technology Department, Chair of Biotechnology, Jamnikarjeva, University of Ljubljana, 101, 1111 Ljubljana, Slovenia;(2) Biotechnical Faculty, Department of Biology, Chair of Animal Physiology, Vena pot 111, 1111, University of Ljubljana, Ljubljana, Slovenia;(3) Department of Environmental Sciences, Joef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia |
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Abstract: | The study presented in this article investigated the influence of different Cr(III) and Cr(VI) compounds in the cultivation medium on the uptake and localization of chromium in the cell structure of the yeast Candida intermedia. The morphology of the yeast cell surface was observed by the scanning electron microscopy. Results demonstrated that the growth inhibitory concentration of Cr(III) in the cultivation medium induced changes in the yeast cell shape and affected the budding pattern, while inhibitory concentration of Cr(VI) did not cause any visible effects on morphological properties of the yeast cells. The amount of total accumulated chromium in yeast cells and the distribution of chromium between the yeast cell walls and spheroplasts were determined by atomic absorption spectroscopy. No significant differences were found neither in total chromium accumulation nor in the distribution of chromium in yeast cell walls and spheroplasts between the two of Cr(VI) compounds. Conversely, substantial differences between Cr(III) compounds were demonstrated in the total uptake as well as the localization of chromium in yeast cells. |
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Keywords: | accumulation chromium distribution morphology yeasts |
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