Ionic strength: A neglected variable in enzyme technology |
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| Authors: | Bruce E. Dale Don H. White |
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| Affiliation: | Department of Agricultural and Chemical Engineering, Colorado State University, Fort Collins, CO 80523, USA;Department of Chemical Engineering, University of Arizona, Tucson, AZ 85721, USA |
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| Abstract: | Ionic strength is a variable of considerable importance in enzyme biochemistry. Unfortunately, the ionic strength variable is little used in immobilized enzyme studies. This paper gives examples of enzyme systems known to be sensitive to ionic strength. Suggestions are offered for the manipulation of ionic strength in enzyme technology studies. Laboratory results on the effect of ionic strength on the reaction catalysed by immobilized pancreatic ribonuclease (EC 3.1.27.5) are also presented and analysed using the Debye-Hückel equation. |
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| Keywords: | Thermodynamics ionic strength enzyme reactions Debye-Hückel theory |
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