Comparison of pectolytic enzymes covalently bound to synthetic ion exchangers using different methods of binding |
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Authors: | Milena Kmínková Jirí Kučera |
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Affiliation: | Research Institute of Food Industry, Na bělidle 21, 150 38 Prague 5, Czechoslovakia |
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Abstract: | Several methods have been compared for the immobilization of a crude commercial pectolytic enzyme product (Pectofoetidin G3X) on derivatives of technical ion exchangers. The conditions were optimized for maximum retention of the enzyme activity and the efficiency of the hydrolysis of pectin solution was measured for all the immobilized enzyme preparations. The merits and drawbacks of the methods used are discussed. |
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Keywords: | Ion exchange methods pectolytic enzymes enzyme immobilization polystyrene |
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