Bioelectrochemical fuel cell and sensor based on a quinoprotein,alcohol dehydrogenase |
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| Authors: | Graham Davis H.Allen O. Hill William J. Aston I. John Higgins Antony P.F. Turner |
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| Affiliation: | Inorganic Chemistry Laboratory, University of Oxford, OX1 3QR, UK;Biotechnology Centre, Cranfield Institute of Technology, Bedford MK43 0AL, UK |
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| Abstract: | A biofuel cell, yielding a stable and continuous low-power output, based on the enzymatic oxidation of methanol to formic acid has been designed and investigated. The homogeneous kinetics of the electrochemically-coupled enzymatic oxidation reaction were investigated and optimized. The biofuel cell also functioned as a sensitive method for the detection of primary alcohols. A method for medium-scale preparation of the enzyme alcohol dehydrogenase [alcohol:(acceptor) oxidoreductase, EC 1.1.99.8] is described. |
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| Keywords: | Methanol biofuel cell sensor alcohol dehydrogenase, EC 1.1.99.8 quinoprotein cyclic voltammetry |
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