Isolation of mutants of Talaromyces emersonii CBS 814.70 with enhanced cellulase activity |
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Authors: | AP Moloney TJ Hackett PJ Considine MP Coughlan |
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Institution: | 1. Department of Biochemistry, University College, Galway, Ireland;2. Institute for Industrial Research and Standards (Biochemical Unit), University College, Galway, Ireland |
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Abstract: | By a combination of genetic mutation and modification of growth medium the cellulase see 1,4-(1,3;1,4)-β-d-glucan 4-glucanohydrolase, EC 3.2.1.4 etc.] activity of culture filtrates of Talaromyces emersonii CBS 814.70 has been increased four-fold to approximately 2 U ml?1 and a productivity of 20–25 Ul?1h?1. At 50°C this system was completely stable for at least 24 h. At 60°C in static reaction mixtures 19% of the original activity was lost compared with 21% when mixtures were shaken. At 70°C the loss of activity after 4 h was 64% without shaking and 70% when shaken. The cellulase system from Trichoderma reesei was decidedly less stable than that of Talaromyces emersonii under each of the above conditions. The ability of each enzyme system, separately and together, to digest beet pulp was investigated. |
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Keywords: | Cellulose cellulase genetic mutation stability beet pulp |
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