| Effects of Huangqi （Hex） on Inducing Cell Differentiation and Cell Death in K562 and HEL Cells |
| |
| 引用本文： | Cheng XD,Hou CH,Zhang XJ,Xie HY,Zhou WY,Yang L,Zhang SB,Qian RL. Effects of Huangqi （Hex） on Inducing Cell Differentiation and Cell Death in K562 and HEL Cells[J]. Acta biochimica et biophysica Sinica, 2004, 36(3): 211-217 |
| |
| 作者姓名： | Cheng XD Hou CH Zhang XJ Xie HY Zhou WY Yang L Zhang SB Qian RL |
| |
| 作者单位： | Xiao-Dong CHENG1,Chun-Hui HOU1,Xue-Jun ZHANG2,Heng-Yue XIE1,Wei-Ying ZHOU1,Lei YANG2,Shu-Bing ZHANG1,and Ruo-Lan QIAN1 State Key Laboratory of Molecular Biology,Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,the Chinese Academy of Sciences,320 Yueyang Road,Shanghai 200031,China; Laboratory of Molecular Cell Biology,Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,the Chinese Academy of Sciences,320 Yueyang Road Shanghai 200031,China the Chinese Academy of Sciences,320 Yueyang Road,Shanghai 200031,China |
| |
| 基金项目： | This work was supported by the grants from the National NaturalScience Foundation of China (No. 39893320,No. 39870378) and theFoundation of the Chinese Academy of Sciences (No. Kj 982-j1618) |
| |
| 摘 要： | InterestintraditionalChineseherbalremedieshasboomedin the western countries. It is very important to study theirmolecular mechanisms and purify effective compoundswith new knowledge and new techniques to meet a greatneed for human health. Ephedrine, the f…
|
| 关 键 词： | 红白血病细胞系 人类 细胞死亡 细胞分化 黄芪 中药 癌症 分子机制 |
Effects of Huangqi (Hex) on inducing cell differentiation and cell death in K562 and HEL cells |
| |
| Cheng Xiao-Dong,Hou Chun-Hui,Zhang Xue-Jun,Xie Heng-Yue,Zhou Wei-Ying,Yang Lei,Zhang Shu-Bing,Qian Ruo-Lan. Effects of Huangqi (Hex) on inducing cell differentiation and cell death in K562 and HEL cells[J]. Acta biochimica et biophysica Sinica, 2004, 36(3): 211-217 |
| |
| Authors: | Cheng Xiao-Dong Hou Chun-Hui Zhang Xue-Jun Xie Heng-Yue Zhou Wei-Ying Yang Lei Zhang Shu-Bing Qian Ruo-Lan |
| |
| Affiliation: | State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China. |
| |
| Abstract: | Huangqi (Astragalus membranaceus), a traditional Chinese medicine, has been used to ameliorate side effects of cancer chemotherapy in China. However, little is known about its molecular mechanisms. Here we show that induction of K562 or HEL cells with 1.5 mg/ml of Huangqi (Hex) (Components extracted from Huangqi) for 3-5 d results in the expression of beta-globin gene in both cell lines and leads to terminal differentiation. Moreover, the apoptosis in HEL cells can be induced by increasing concentration of Huangqi (Hex) to 4.5 mg/ml for 3-5 d. Upregulation of Apaf-1, caspase-3 and acetylcholinesterase (AChE) in HEL cells may play a crucial role in the process of apoptosis. The prospect of inducing expression of adult (beta) globin gene and apoptosis selectively in cancer cells is obviously attractive from a therapeutic point of view. |
| |
| Keywords: | cell death and differentiation Huangqi (Hex) human erythroleukemia cell lines |
| 本文献已被 CNKI 维普 PubMed 等数据库收录！ |
|
