Uptake and metabolism of radioactive gibberellins by barley aleurone layers |
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Authors: | Alan Musgrave Sandra E Kays Hans Kende |
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Institution: | (1) MSU/AEC Plant Research Laboratory, Michigan State University, East Lansing;(2) Unit of Developmental Botany Agricultural Research Council, University of Cambridge, CB3 ODY Cambridge, UK |
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Abstract: | Summary When aleurone layers were treated with labeled gibberellin A1 (3H-GA1), gibberellin A5 (3H-GA5) and the methyl ester of 3H-GA5 (3H-GA5-ME), radioactivity was accumulated by the tissue for a period of 20–30 h. After this time, radioactivity was released into the medium. Concomitantly, ribonuclease was also liberated by the tissue. The radioactivity accumulated by aleurone layers was associated with polar metabolites of the respective GAs, and the extent of extent of accumulation was a function of the degree of GA metabolism (GA5-ME>GA5>GA1). Accumulation of radioactivity was inhibited in the cold and by the metabolic poisons NaF and dinitrophenol. This was thought to be due to inbition of GA metabolism. The accumulation of 3H-GA1 in aleurone tissue did not reach saturation when unlabeled GA3 up to 10-2 M was added to the incubation medium.Abbreviations GA
gibberellin
- GA5
ME, gibberellin A5 methyl ester
- RNase
ribonuclease |
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