Evaluation of two alternative metabolic pathways for creatinine degradation in microorganisms |
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Authors: | Sakayu Shimizu Jong Min Kim Yoshifumi Shinmen Hideaki Yamada |
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Affiliation: | (1) Department of Agricultural Chemistry, Kyoto University, 606 Kyoto, Japan |
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Abstract: | The microbial decomposition of creatinine was found to proceed mainly via N-methylhydantoin or creatine as the first degradation product. Either N-methylhydantoin or urea or both were detected as metabolites derived from creatinine in various microorganisms, and creatinine deiminase and creatinine amidohydrolase activities were detected concomitantly. N-Methylhydantoin hydrolase and N-carbamoylsarcosine amidohydrolase were found to be formed inducibly in the presence of creatinine or N-methylhydantoin. Three microorganisms which decompose creatinine in different ways were screened from soil. Pseudomonas putida 77 rapidly metabolized creatinine solely via N-methylhydantoin. Degradation of creatinine proceeded with both creatine and N-methylhydantoin as the first degradation products at the same time in Pseudomonas sp. H21. Pseudomonas sp. 0114 was found to metabolize creatinine mainly via creatine and to also metabolize N-methylhydantoin. Changes in the metabolites of creatinine during a cultivation or enzyme reaction were found to be closely related to the enzyme activities of interest which are regulated by creatinine or N-methylhydantoin in different ways depending on the microbial strain. |
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Keywords: | Creatinine degradation Pseudomonas putida 77 Pseudomonas sp. H21 Pseudomonas sp. 0114 N-Methylhydantoin degradation Creatinine deiminase N-Methylhydantoin hydrolase N-Carbamoylsarcosine amidohydrolase Creatine amidinohydrolase |
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