Action of insulin on liver carbamoyl-phosphate synthetase II (glutamine-hydrolyzing) activity |
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Authors: | M A Reardon G Weber |
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Institution: | 1. The Research Service, Veterans Administration Medical Center, Nashville, Tennessee 37203 USA;2. the Department of Medicine (Dermatology), Vanderbilt University School of Medicine Nashiville, Tennessee 37203 USA |
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Abstract: | Carbamoyl-phosphate synthetase II (glutamine-hydrolyzing) (EC 6.3.5.5) (synthetase II), is the first and rate-limiting enzyme in the de novo UMP biosynthetic pathway. The present investigation showed that insulin has a regulatory action on hepatic synthetase II activity. When diabetes was induced with injection of different doses of alloxan the plasma insulin concentrations decreased in a dose-dependent fashion to 72, 38, 31 and 28% and concurrently the liver synthetase II activity decreased to 75, 43, 29 and 22% of the normal values. In diabetic rats dose response studies showed that with insulin injections of 4, 6, 8 or 10 U/day for 48 h the hepatic synthetase II activity increased to 81, 95, 99 and 103% of the control liver values. In the diabetic rats the insulin-induced rise in liver synthetase II activity was prevented by treatment of the rats with actinomycin. |
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Keywords: | EGF epidermal growth factor EGTA ethyleneglycol-bis-(β-aminoethyl ether)NN′-tetraacetic acid SDS sodium dodecyl sulfate PAGE polyacrylamide gel electrophoresis HEPES 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid CANP calcium-activated neutral protease |
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