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High-yield production of mannitol by <Emphasis Type="Italic">Leuconostoc pseudomesenteroides</Emphasis> CTCC G123 from chicory-derived inulin hydrolysate
Authors:Min Zhang  Lei Gu  Chao Cheng  Junru Zhu  Hao Wu  Jiangfeng Ma  Weiliang Dong  Xiangping Kong  Min Jiang  Pingkai Ouyang
Institution:1.State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM),Nanjing Tech University,Nanjing,People’s Republic of China
Abstract:Chicory is an agricultural plant with considerable potential as a carbohydrate substrate for low-cost production of biochemicals. In this work, the production of mannitol by Leuconostoc pseudomesenteroides CTCC G123 from chicory-derived inulin hydrolysate was investigated. The bioconversion process initially suffered from the leakage of fructose to the phosphoketolase pathway, resulting in a low mannitol yield. When inulin hydrolysate was supplemented with glucose as a substrate for mannitol production in combination with aeration induction and nicotinic acid induced redox modulation strategies, the mannitol yield greatly improved. Under these conditions, significant improvement in the glucose consumption rate, intracellular NADH levels and mannitol dehydrogenase specific activity were observed, with mannitol production increasing from 64.6 to 88.1 g/L and overall yield increase from 0.69 to 0.94 g/g. This work demonstrated an efficient method for the production of mannitol from inulin hydrolysate with a high overall yield.
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