Evolution of copper arsenate resistance for enhanced enargite bioleaching using the extreme thermoacidophile <Emphasis Type="Italic">Metallosphaera sedula</Emphasis> |
| |
| Authors: | Chenbing Ai Samuel McCarthy Yuting Liang Deepak Rudrappa Guanzhou Qiu Paul Blum |
| |
| Institution: | 1.School of Minerals Processing and Bioengineering,Central South University,Changsha,People’s Republic of China;2.School of Biological Sciences, Beadle Center for Genetics,University of Nebraska-Lincoln,Lincoln,USA |
| |
| Abstract: | Adaptive laboratory evolution (ALE) was employed to isolate arsenate and copper cross-resistant strains, from the copper-resistant M. sedula CuR1. The evolved strains, M. sedula ARS50-1 and M. sedula ARS50-2, contained 12 and 13 additional mutations, respectively, relative to M. sedula CuR1. Bioleaching capacity of a defined consortium (consisting of a naturally occurring strain and a genetically engineered copper sensitive strain) was increased by introduction of M. sedula ARS50-2, with 5.31 and 26.29% more copper recovered from enargite at a pulp density (PD) of 1 and 3% (w/v), respectively. M. sedula ARS50-2 arose as the predominant species and modulated the proportions of the other two strains after it had been introduced. Collectively, the higher Cu2+ resistance trait of M. sedula ARS50-2 resulted in a modulated microbial community structure, and consolidating enargite bioleaching especially at elevated PD. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
